lentiviral dcas9 krab expression construct (Addgene inc)
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Addgene inc
lentiviral dcas9 krab expression construct
Lentiviral Dcas9 Krab Expression Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+expression+plasmids+constructs/pLV+hU6-sgRNA+hUbC-dCas9-KRAB-T2a-Puro+(Plasmid+%2371236)/pm42868712-126-1-5
Average 96 stars, based on 210 article reviews
Lentiviral Dcas9 Krab Expression Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sgrna+expression+plasmids+constructs/pLV+hU6-sgRNA+hUbC-dCas9-KRAB-T2a-Puro+(Plasmid+%2371236)/pm42868712-126-1-5
Average 96 stars, based on 210 article reviews
lentiviral dcas9 krab expression construct - by Bioz Stars,
2026-10
96/100 stars
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Expressing:Article Title: Targeting specific DNA G-quadruplexes with CRISPR-guided G-quadruplex-binding proteins and ligands. Article Snippet: Despite the demonstrated importance of DNA G-quadruplexes (G4s) in health and disease, technologies to readily manipulate specific G4 folding for functional analysis and therapeutic purposes are lacking.. Here we employ G4-stabilizing protein/ligand in conjunction with CRISPR to selectively facilitate single or multiple targeted G4 folding within specific genomic loci.. We demonstrate that fusion of nucleolin with a catalytically inactive Cas9 can specifically stabilize G4s in the promoter of oncogene MYC and muscle-associated gene Itga7 as well as telomere G4s, leading to cell proliferation arrest, inhibition of myoblast differentiation and cell senescence, respectively. Construct:Article Title: Targeting specific DNA G-quadruplexes with CRISPR-guided G-quadruplex-binding proteins and ligands. Article Snippet: Despite the demonstrated importance of DNA G-quadruplexes (G4s) in health and disease, technologies to readily manipulate specific G4 folding for functional analysis and therapeutic purposes are lacking.. Here we employ G4-stabilizing protein/ligand in conjunction with CRISPR to selectively facilitate single or multiple targeted G4 folding within specific genomic loci.. We demonstrate that fusion of nucleolin with a catalytically inactive Cas9 can specifically stabilize G4s in the promoter of oncogene MYC and muscle-associated gene Itga7 as well as telomere G4s, leading to cell proliferation arrest, inhibition of myoblast differentiation and cell senescence, respectively. Plasmid Preparation:Article Title: Targeting specific DNA G-quadruplexes with CRISPR-guided G-quadruplex-binding proteins and ligands. Article Snippet: Despite the demonstrated importance of DNA G-quadruplexes (G4s) in health and disease, technologies to readily manipulate specific G4 folding for functional analysis and therapeutic purposes are lacking.. Here we employ G4-stabilizing protein/ligand in conjunction with CRISPR to selectively facilitate single or multiple targeted G4 folding within specific genomic loci.. We demonstrate that fusion of nucleolin with a catalytically inactive Cas9 can specifically stabilize G4s in the promoter of oncogene MYC and muscle-associated gene Itga7 as well as telomere G4s, leading to cell proliferation arrest, inhibition of myoblast differentiation and cell senescence, respectively. |